Proteinase K

Product overview

Protease K is a serine protease with high enzymatic activity and wide substrate specificity. It can preferentially decompose ester and peptide bonds adjacent to the ends of hydrophobic amino acids, sulfur-containing amino acids and aromatic amino acids C, and is often used to degrade proteins to produce short peptides. It has the typical catalytic triad Asp39-His69-Ser224 characteristic of serine proteases and two Ca²⁺ binding sites around the active center increase its stability, allowing it to maintain high enzyme activity under a wider range of conditions.

Technical index

Appearance: Colorless to light brown liquid; Specific activity: ≥800 U/mL; Protein concentration: ≥20 mg/mL

Enzymatic property

Source: Tritirachiumalbum; Category: EC 3.4.21.64; Molecular weight: 29 kDa(SDS-PAGE); Isoelectric point: 7.81; The optimal pH: 7.0-12.0 has high activity; Optimum temperature: 65℃; pH stability: pH 4.5-12.5(25℃,16 h); Thermal stability: stable below 50℃ (pH 8.0,30 min); Storage stability: 25℃ a year activity of more than 90% activator SDS, urea; Inhibitors: diisopropyl fluorophosphate (DFIP), benzoyl fluoride (PMSF); Storage conditions: 2-8℃, valid for 24 months.

use

1. Genetic diagnostic kit;
2. RNA and DNA extraction kit;
3. Extraction of non-protein components from tissues, degradation of protein impurities, such as DNA vaccine and heparin preparation;
4. Preparation of chromosome DNA by pulsed electrophoresis;
5. Western blot;
6. Research and development and mass production of enzymatic glycosylated albumin reagents for in vitro diagnosis.

Matters needing attention

Wear protective gloves and goggles when using, and keep well ventilated after use. This product may cause allergic skin reactions.