resDNASEQ E1A&SV40 Residual DNA Quantitation kit
$499.00
This kit is based on the fluorescent quantitative PCR method, and primer probes are designed for the E1A gene and SV40LTA gene. A rapid detection and high sensitivity real-time fluorescent quantitative PCR kit is established. It is a special kit for quantitatively detecting E1A&SV40LTA residual DNA from host cells, such as 293T cells, in biological products. This kit is used in conjunction with DuckyBio Host Cell praparation Kit, and can accurately quantify trace E1A&SV40LTA DNA in samples in as fast as 1.5 hours.
| Catalog number: | Cat. No. D24011210 |
|---|---|
| Qty: | 50 Reactions |
Description
HEK293T is a very common expression vector in cell therapy, gene therapy and biopharmaceuticals, and is widely used in the production of proteins, vaccines, anticancer agents and recombinant adenovirus coatings. HEK 293T cells carry specific transformation sequences that are known to have potential safety risks, such as Adv E1A sequences and SV40 large T antigen sequences. Therefore, relevant regulations require that when HEK293T cells are used to prepare lentiviral vectors in products, a method with sufficient sensitivity and specificity must be used to detect the residues of adenovirus E1 and SV40LT antigen sequences.
Features of the sDNASEQÂ E1A&SV40 Residual DNA Quantitation kit include:
Simpler and Rapid
- Only three steps will be need for Sample Preparation and All components of the Sample Preparation Kit can be stored at room temperature.
- Only one Reagent for qPCR;
- Only 1.5 hours will be needed for the whole test.
Accurate
- Perfect amplification curve, good amplification efficiency and good precision.
- Highly sensitive quantitation using proven TaqManâ„¢ real-time qPCR technology.
- E1A Limit of Detection (LOD): 0.4 copies/μL; E1A Limit of Quantification (LOQ): 4 copies/μL.
- SV40 Limit of Detection (LOD):0.4 copies/μL; SV40 Limit of Quantification (LOQ): 4 copies/μL.
- The recovery rate of different concentration samples in the linear range is between 70% and 130%
Kit Performance
Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.
Fig 2. All components of Sample Preparation Kit can be stored at room temperature.
