Problem Cause Recommendation Fluorescence intensity varies. Some of the reagent is still in the upper part of the microwell or an air bubble is trapped in the microwell. Repeat centrifugation, but allow sufficient centrifugation time so all reagent is at the bottom of the microwell and air bubbles are expelled. Skin oils or dirt on […]
Good laboratory practices Before You Begin Good laboratory practices for PCR and RT-PCR Precautions: To avoid contamination, perform the workflow setup under DNA-free conditions. This includes: Prepare and pipette all solutions with nuclease-free, DNA-free equipment and consumables. UV-treat or the laminar flow hood prior to pipetting. Use sterile single-use gloves and freshly laundered laboratory coats. Change gloves if
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